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KMID : 1025520090510050369
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Journal of Animal Science and Technology
2009 Volume.51 No. 5 p.369 ~ p.378
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Protein Fractionation of Whole Crop Silages, and Effect of Borate-phosphate Buffer Extraction on In vitro Fermentation Characteristics, Gas Production and Degradation
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Shinekhuu Jugdder
Jin Guang-Lin
Ji Byung-Ju
Li Xiang-Zi
Oh Young-Kyoon
Hong Seong-Koo
Song Man-Kang
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Abstract
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Protein fractionation was evaluated from whole crop silages of rye(RS), wheat(WS), triticale(TS), oat(OS), barley(BS), and rice straw silage(RSS), and in vitro trial was carried out to examine the effect of silage and extraction of soluble protein on fermentation characteristics, total gas production and degradation. Soluble protein of silages was extracted with borate-phosphate buffer, and fermentation characteristics, gas production and degradation of silages were estimated by incubating anaerobically the mixed solution of strained rumen fluid and artificial saliva(1:1, v/v) containing dried and ground silages placed in nylon bag at 39℃ up to 48h. Soluble protein(SP) content was lowest for RSS as 2.11% in total CP compared to those for other silages. Highest A fraction(NPN) was observed from RS (74.33% of total CP) while those from TS and RSS were relatively low(48%). B2 fraction was relatively higher for RS, RSS and WS than for TS and BS. B3 fraction was lowest in WS among silages. C fraction(27.07) in RSS was higher than in other silages(1.40∼9.93%). pH in incubation solution was increased(P<0.01∼P<0.001) for extracted silages up to 12h but decreased(P<0.01) at 48h for non-extracted ones. Contents of ammonia-N(P<0.001) and total VFA(P<0.01∼P<0.001) were higher for non-extracted silages than for extracted ones. Acetate proportion was increased(P<0.001) in buffer extracted silages while those of propionate and butyrate were decreased(P<0.001) up to 24h incubation. Increased (P<0.001) total gas production was obtained from non-extracted silages up to 12h while gas production was increased(P<0.01) in extracted ones thereafter. In vitro degradation of dry matter and CP was increased(P<0.001) in non-extracted silages but that of neutral detergent fiber was increased(P<0.001) in extracted ones without difference among silages. Difference in mean values of degradability for each silage prior to- and post extraction with borate buffer, however, was not found among silages. It may be concluded that high NPN content of silages may reduce the protein availability in silages and borate buffer soluble components in silages can stimulate the early stage of fermentation.
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KEYWORD
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Whole crop silages, Protein fractionation, Fermentation, Gas production, In vitro degradation
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